بررسی بیان ژن یوبیکوئیتین D در انواع سرطان خون

Background:Research has shown that the expression of UBD (Ubiquitin D) is effective in causing cancer. UBD relationship between states with advanced stages of cancer cell differentiation and expression, which probably UBD involved in the progression of carcinogenesis. Overexpression of UBD is related to p53, which subsequently progress to cancer. The purpose of this study is evaluation of Ubiquitin D gene expression in leukemia patient as a biomarker for detection of disease and its progression in patients as well. Methods: In this study 100 patients with leukemia blood samples and 100 blood samples from healthy people between ages 36 to 74 were collected. RNA is then extracted and cDNA synthesis to enzyme MMuLV and Random hexamer primers Oligo dt was done. UBD specific primers were designed using Primer Express software and gene expression levels UBD by ΔΔct (relative) were measured. Results: UBD average increase gene expression in individuals less than 50 years equal to 5.82, P <0.0001 and in more than is 50 years is 2.10, P <0.0001. 1.70 the lowest expression in CML and in ALL highest expression 7.97 than their on normal average and the mean UBD gene overexpression in AML patients in this group of CLL equal to 3.21 and 4.94 (p-value <0.0001) was observed against. Conclusion: UBD can be examined until perhaps as a biomarker for diagnosis of leukemia proposed to be used. So, we can evaluate the expression of the genes used to diagnose leukemia. However, more studies than the current application procedures, in cancer that has a lot of technical errors that were used in this study and prove its accuracy and sensitivity is required.